| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | A | Pines Elliott | Bullock | Anderson | Daniels | 42.69 | |
| 2 | Lebanon Valley |
A | Goundie | Murtha | Stankus | Winters | 42.47 |
| 3 | Delaware Valley |
A | Madison | Taveras | O'Dunlami | Williams | 42.24 |
| 4 | Misericordia |
A | Blackwell | Billings | Vacchio | Hunter | 41.97 |
| 5 | Eastern |
A | Ugorji | Bateman | Boddie | Greenwood | 42.45 |
| 6 | York (Pa.) |
A | Hardison | Bonham | Diallo | Moore | 42.29 |
| 7 | A | Jones | Gorski | Novrocki | Mullery | 42.63 | |
| 8 | Widener |
A | Grossman | Wallace | Knowles | Ndirangu | 42.97 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Albright |
A | Ruffin | Edwards | Maley | Hammond | 45.46 | Purple & Gold Open |
| 2 | DeSales |
A | Guthier | Estherson | Jacobson | McCulloch | 43.68 | DeSales Quad Meet |
| 3 | Widener |
A | Grossman | Ressler | Knowles | Ndirangu | 42.14 | 2025 Paul Donahue Invitational at Widener University |
| 4 | A | Wagner | Sorrentino | Perrelli | Evely | 44.01 | 2025 Kings College Monarch Open | |
| 5 | Eastern |
A | Ugorji | Bateman | Boddie | Greenwood | 42.23 | Danny Curran Invite |
| 6 | A | Pines Elliott | Bullock | Anderson | Daniels | 42.53 | Penn Relays | |
| 7 | A | Jones | Gorski | Novrocki | Mullery | 42.36 | 2025 Kings College Monarch Open | |
| 8 | Messiah |
A | Karyea | Grove | Sobek | Lewis | 45.64 | 20th Annual Paul Kaiser Classic |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Delaware Valley |
A | Madison | Taveras | O'Dunlami | Williams | 42.79 | Danny Curran Invite |
| 2 | Stevens |
A | Hackett | Savino | Robinson | Winant | 42.61 | 2025 Coach P Invite |
| 3 | Misericordia |
A | Blackwell | Billings | Vacchio | Hunter | 42.26 | Penn Relays |
| 4 | York (Pa.) |
A | Hardison | Bonham | Diallo | Moore | 42.29 | 20th Annual Paul Kaiser Classic |
| 5 | Lebanon Valley |
A | Goundie | Murtha | Stankus | Winters | 42.00 | 2025 Messiah Multi/Invitational |
| 6 | Alvernia |
A | Washington | Simmons | Downs | Marcin | 44.43 | York College Twilight |
| 7 | Stevenson |
A | Parker | McCann | Abdur-Rahman | Hammond | 44.11 | Towson Invitational |