| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | A | Zultevicz | Butcher | Springer | Thompson | 50.64 | |
| 2 | A | Jones | Marcinkiewicz | Kowalski | Barnett | 50.01 | |
| 3 | DeSales |
A | Carrier | Ribeiro | Peller | Signorella | 49.64 |
| 4 | Widener |
A | Muzzarelli | Robinson | Gore | Koita | 47.39 |
| 5 | Lebanon Valley |
A | Berkheimer | Matter | Reilly | Calderon | 49.43 |
| 6 | York (Pa.) |
A | Mann | Boyce | Nuse | Lopez | 49.88 |
| 7 | Messiah |
A | Brown | Graybill | Benner | Gruenwald | 50.58 |
| 8 | Eastern |
A | Jefferson | Majett | Cooper | Johnson | 50.70 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | DeSales |
A | Carrier | Ribeiro | Peller | Signorella | 51.20 | Danny Curran Invite |
| 3 | Eastern |
A | Jefferson | Majett | Cooper | Dixon | 50.89 | Danny Curran Invite |
| 4 | A | Zultevicz | Butcher | Springer | Thompson | 50.20 | 2025 Kings College Monarch Open | |
| 5 | Lebanon Valley |
A | Berkheimer | Matter | Reilly | Calderon | 49.15 | 20th Annual Paul Kaiser Classic |
| 6 | Stevenson |
A | Day | Stephens | Brady | Fowler | DQ | York College Twilight |
| 7 | Messiah |
A | Brown | Graybill | Benner | Gruenwald | 53.62 | 20th Annual Paul Kaiser Classic |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Widener |
A | Muzzarelli | Robinson | Gore | Koita | 48.21 | Penn Relays |
| 3 | York (Pa.) |
A | Mann | Boyce | Nuse | Lopez | 50.37 | 20th Annual Paul Kaiser Classic |
| 4 | Stevens |
A | Alley | Canciani | Sadowski | Tighe | 51.59 | 2025 Coach P Invite |
| 5 | Alvernia |
A | Wallace | Gularte | Rodriguez | Schwarz | 53.16 | York College Twilight |
| 6 | A | Jones | Marcinkiewicz | Kowalski | Barnett | 50.81 | 2025 Paul Donahue Invitational at Widener University |