| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Messiah |
A | Lebo | Kimmel | Kime | Pineda | 10:06.35 | 2025 Messiah Multi/Invitational |
| 2 | York (Pa.) |
A | Reidenbaugh | Seymour | Brass | Tenney | 10:39.04 | York College Twilight |
| 3 | Lebanon Valley |
A | Kapcsos | Hunt | Nemitz | Garges | 10:47.63 | 2025 Messiah Multi/Invitational |
| 4 | Widener |
A | Golembiewski | Schroeder | O'Brien | Sweely | NT | |
| 5 | Stevens |
A | Gabay | Enes | Burden | Vogel | NT | |
| 6 | Stevenson |
A | Brady | Byers | Lacey | Davis | NT | |
| 7 | Delaware Valley |
A | Herrera | East | Swearingen | Gechter | NT | |
| 8 | A | Johnson | Adams | Eyer | Chipriano | NT | ||
| 9 | Eastern |
A | Croge | Cadena | Ferrara | Shreffler | NT |