| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 4 | Alvernia |
A | Schwarz | Sternberg | McCloud | Wallace | NT | |
| 5 | A | Zultevicz | Butcher | Hahn | Springer | 1:55.66 | 1:55.66 PLEX Shootout |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | York (Pa.) |
A | Bankert | Mann | Tenney | Boyce | 1:55.60 | 1:55.60 PLEX Shootout |
| 4 | A | Marcinkiewicz | Kowalski | Jones | Smilovic | DNF | 1:52.13 River Hawk Friday Night Showdown | |
| 5 | Stevens |
A | Shanley | Ryazanova | Sadowski | Alley | 1:51.88 | 1:51.88 PLEX Shootout |
| 6 | DeSales |
A | Peluszak | Wiscount | Peller | Ribeiro | 1:53.30 | 1:53.30 DeSchriver |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Misericordia |
A | Krevey | Grasso | Doyle | Iglesias | 1:51.29 | 1:51.29 Ithaca Bomber Invitational |
| 3 | Messiah |
A | Gruenwald | Jennett | Benner | Brown | DNF | 1:51.09 Alvernia Winter Invitational |
| 4 | Lebanon Valley |
A | Matter | Berkheimer | Reilly | Calderon | 1:47.80 | 1:47.80%25 %281:46.15%29 Scarlet Knight Open |
| 5 | Widener |
A | Muzzarelli | Thomas | Kolimaga | Koita | 1:46.13 | 1:46.13 Alvernia Winter Invitational |
| 6 | Eastern |
A | Jefferson | Majett | Johnson | Anderson | 1:50.44 | 1:50.44 Keogh Invitational 2025 |