| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | DeSales |
A | McCulloch | Guthier | Marsh | Callano | NT | |
| 4 | A | Sorrentino | Presley | Perrelli | Evely | 1:39.93 | 1:39.93 PLEX Shootout | |
| 5 | Albright |
A | Myers | Maley | Edwards | Ruffin | DQ | 1:39.39 Seamus McElligott Invitational 2025 |
| 6 | Stevenson |
A | Reimert | McCann | Cherenfant | Saint Hillien | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | A | Mullery | Clarke | Gorski | Novrocki | 1:35.68 | 1:35.68 PLEX Shootout | |
| 3 | Stevens |
A | Hackett | Kosiba | Ladd | Robinson | DQ | 1:35.00 PLEX Shootout |
| 4 | York (Pa.) |
A | Hardison | Moore | Bonham | Diallo | 1:34.09 | 1:34.09 PLEX Shootout |
| 5 | Alvernia |
A | St. Pierre | Downs | Simmons | Auzenne | 1:33.72 | 1:33.72 PLEX Shootout |
| 6 | Delaware Valley |
A | Madison | Taveras | O'Dunlami | Williams | 1:34.11 | 1:34.11 PLEX Shootout |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Lebanon Valley |
A | Goundie | Murtha | Bruchak | Grimm | 1:33.60 | 1:33.60 SU Tune Up |
| 3 | Widener |
A | Ressler | McGraw | Celestin | Knowles | 1:33.33 | 1:33.33 Seamus McElligott Invitational 2025 |
| 4 | Eastern |
A | Ugorji | Franklin | Howard | Greenwood | 1:32.90 | 1:32.90 Seamus McElligott Invitational 2025 |
| 5 | Misericordia |
A | Billings | Blackwell | Vacchio | Hunter | 1:31.26 | 1:31.26 Ithaca Bomber Invitational |
| 6 | A | Daniels | Carroll | Castelli | Bullock | 1:32.99 | 1:32.99 Alvernia Winter Invitational |