| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Susquehanna Township | A | Sweat | Strawbridge | Mathes | Leaks | DNS | Susquehanna Township vs. Mechani |
| 4 | Harrisburg Christian | A | Rudolph | Stargel | Sunday | Shram-Groff | 3:51.38 | Harrisburg HS vs Central Dauphin |
| 5 | Lower Dauphin | A | Reza | Persing | Soriano | Grow | 4:02.06 | Lower Dauphin HS vs. Milton Hers |
| 6 | Lower Dauphin | B | 4:02.06 | Lower Dauphin HS vs. Milton Hers |