| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Lower Dauphin | A | Everett | Shradley | Persing | Gidusko | 47.46 | Lower Dauphin HS vs. Milton Hers |
| 3 | Susquehanna Township | A | Gault, Jr. | Brown | Mathes | Leaks | 43.48 | Susquehanna Township vs. Mechani |
| 4 | Harrisburg Christian | A | Horst | Mack | Horst | Savage | 49.64 | Harrisburg HS vs Central Dauphin |