| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Lower Dauphin | A | Debock | Reeder | Bean | Eismann | 54.12 | Lower Dauphin HS vs. Milton Hers |
| 3 | Susquehanna Township | A | Coates | Oliseh | Archer | Dorsey | 50.58 | Susquehanna Township vs. Mechani |
| 4 | Harrisburg Christian | A | Rudolph | Idowu | Gantz | Masters | 59.24 | Harrisburg HS vs Central Dauphin |