| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Central Dauphin East | B | DNS | |||||
| 2 | Harrisburg | A | DNS | MC/Harrisburg @ Altoona | ||||
| 3 | Central Dauphin East | A | 3:43.07 | Tim Cook Invitational | ||||
| 4 | Cumberland Valley | A | 3:26.93 | Bruce Dallas Invitational | ||||
| 5 | Cumberland Valley | B | 3:26.93 | Bruce Dallas Invitational | ||||
| 6 | Harrisburg | B | DNS | MC/Harrisburg @ Altoona |