| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MITCHELL, Addison | FR | Altoona Area | 50.24 | |
| 2 | SETUAGBE, Sefa | SR | Cumberland Valley | 48.11 | State College vs Cumberland Vall |
| 3 | WARFEL, Danielle | SR | Mifflin County | NT | |
| 4 | IRWIN, Alannah | SR | Altoona Area | 45.99 | PIAA District 6 AAA Championship |
| 5 | BENINI, Kendall | SO | Cumberland Valley | 51.97 | State College vs Cumberland Vall |
| 6 | BAUMGARDNER, Madison | FR | Mifflin County | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LONG, Grace | JR | Altoona Area | 55.23 | 27th Annual LHU High School Clas |
| 2 | CHAMBERS, Mirielle | FR | Cumberland Valley | 54.5h | |
| 3 | RIDEN, Abigail | FR | Mifflin County | NT | |
| 4 | PUTT, Mataya | SO | Cumberland Valley | 54.83 | Carlisle vs. Cumberland Valley |
| 5 | PATEL, Shanaya | SO | Cumberland Valley | 55.05 | Carlisle vs. Cumberland Valley |
| 6 | SHEETZ, Reagan | FR | Mifflin County | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | SERAN, Daphne | FR | Cumberland Valley | NT | |
| 3 | ABDALLA, Sara | JR | Cumberland Valley | 55.0h | |
| 4 | ABBED, Marwa | SO | Cumberland Valley | NT |