| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BUSH, Camden | FR | Altoona Area | NT | |
| 2 | KOSOFSKY, Owen | JR | Cumberland Valley | 52.94 | Mid Penn Championship |
| 3 | TREASTER, Camryn | SO | Mifflin County | 56.18 | PIAA District 6 AAA Championship |
| 4 | VERTICELLI, Dominic | SO | Altoona Area | 54.81 | Altoona Mt. Lion Track & Field C |
| 5 | HERTZLER, Danny | SO | Cumberland Valley | 53.30 | Bruce Dallas Invitational |
| 6 | DASHEM, Zaiden | JR | Mifflin County | 1:01.73 | Mifflin County/State College @ A |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DIVEN, Trevan | JR | Mifflin County | NT | |
| 2 | COLONNA, Jackson | SR | Cumberland Valley | 54.3h | |
| 3 | YETTER, Winston | JR | Mifflin County | NT | |
| 4 | SINGU, Sohan | SO | Cumberland Valley | 57.29 | State College vs Cumberland Vall |
| 5 | STOLTZ, Ethan | FR | Altoona Area | NT |