| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Hood |
A | 4:32.87 | Danny Curran Invite |
| 3 | A | 4:21.86 | Danny Curran Invite | |
| 4 | Alvernia |
A | 4:20.47 | York College Twilight |
| 5 | A | 4:21.74 | Misericordia Twilight 2025 | |
| 6 | Delaware Valley |
A | 4:31.72 | Danny Curran Invite |
| 7 | Stevenson |
A | 4:34.31 | Hopkins Loyola Invitational |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Messiah |
A | 4:15.10 | Penn Relays |
| 2 | Lebanon Valley |
A | 4:11.99 | York College Twilight |
| 3 | Widener |
A | 4:08.74 | 2025 Paul Donahue Invitational at Widener University |
| 4 | Misericordia |
A | 4:01.15 | Penn Relays |
| 5 | York (Pa.) |
A | 4:08.03 | 20th Annual Paul Kaiser Classic |
| 6 | Eastern |
A | 4:11.47 | Hopkins Loyola Invitational |
| 7 | DeSales |
A | 4:14.27 | Danny Curran Invite |
| 8 | Stevens |
A | 4:16.25 | AARTFC Indoor Track & Field Championships |