| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 398 | KREVEY, Emily | FR | Misericordia |
1:00.64 | Bison Outdoor Classic 2025 |
| 3 | 783 | BURKE, Bianca | JR | 1:00.94 | Ramapo Track & Field Invitational | |
| 4 | 685 | EAST, Emma | SO | Delaware Valley |
1:01.65 | Miller Sports Construction Blue Jay Tune-Up |
| 5 | 840 | BUTCHER, Ava | SO | 59.16 | Danny Curran Invite | |
| 6 | 80 | JENNETT, Halle | SR | Messiah |
58.53 | 20th Annual Paul Kaiser Classic |
| 7 | 694 | PRESCOTT, Zariah | JR | Delaware Valley |
1:01.46 | Ursinus Track & Field Festival |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 736 | ANDERSON, Anahrie | SO | Eastern |
1:00.57 | Miller Sports Construction Blue Jay Tune-Up |
| 3 | 992 | FOWLER, Dallas | FR | Stevenson |
1:02.80 | Hopkins Loyola Invitational |
| 4 | 187 | SCHULTZ, Emma | SR | Widener |
59.92 | 2025 Paul Donahue Invitational at Widener University |
| 5 | 605 | BOYCE, Keziah | JR | York (Pa.) |
1:01.08 | Danny Curran Invite |
| 6 | 877 | BERKHEIMER, Darrian | SR | Lebanon Valley |
1:01.39 | 2025 Messiah Multi/Invitational |
| 7 | 48 | SHANLEY, Isabella | SO | Stevens |
58.29 | Danny Curran Invite |
| 8 | 905 | MILLER, Emily | JR | Lebanon Valley |
1:03.52 | 42nd Millersville Metrics |