| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | DeSales |
A | 51.20 | Danny Curran Invite |
| 3 | Eastern |
A | 50.89 | Danny Curran Invite |
| 4 | A | 50.20 | 2025 Kings College Monarch Open | |
| 5 | Lebanon Valley |
A | 49.15 | 20th Annual Paul Kaiser Classic |
| 6 | Stevenson |
A | 53.22 | York College Twilight |
| 7 | Messiah |
A | 53.62 | 20th Annual Paul Kaiser Classic |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Widener |
A | 48.21 | Penn Relays |
| 3 | York (Pa.) |
A | 50.37 | 20th Annual Paul Kaiser Classic |
| 4 | Stevens |
A | 51.59 | 2025 Coach P Invite |
| 5 | Alvernia |
A | 53.16 | York College Twilight |
| 6 | A | 50.81 | 2025 Paul Donahue Invitational at Widener University |