| R | Bruce Dallas T&F Invitational | 4:00.82 | C.Heilman, A.Pines, M.Boardman, R.Ebersole | Cumberland Valley | 4/2/2022 | 
| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | |
|---|---|---|---|---|---|---|---|---|
| 1 | Cumberland Valley | A | Graziano | Dwyer | Graziano | Robertson | 3:59.0h | |
| 2 | Conestoga Valley | A | Frey | Miller | Fisher | Johnson | 4:07.0h | |
| 3 | Susquenita | A | Pieper | Wechsler | Egresits | Foster | 4:10.91 | Tim Cook Invitational | 
| 4 | Lewisburg Area | A | Batkowski | Osunde | Schwartz | Moyers | 4:13.78 | Lewisburg & Montoursville at Mid | 
| 5 | William Penn | A | Bailey | Bradshaw-Moran | Smith | Kleinfield | 4:17.00 | |
| 6 | Wilson 03 | A | Reidinger | Levandowski | Houck | Weyman | 4:17.61 | Tim Cook Invitational | 
| 7 | South Western | A | Arnold | Glover | Peake | Shulski | 4:21.24 | Spring Grove | 
| 8 | Carlisle | A | 4:21.88 | |||||
| 9 | Central Dauphin East | A | Premici | Turner | Robinson | Livingston | 4:22.80 | Tim Cook Invitational | 
| 10 | Susquehanna Township | A | Coates | Crosson | King | Dorsey | 4:27.83 | Lower Dauphin HS vs. Susquehanna | 
| 11 | Midd-West | A | Faulhaber | Knepp | Treaster | Stroup | 4:28.68 | Lewisburg & Montoursville at Mid | 
| 12 | Harrisburg | A | Bryant | Corbitt | Harrison | Hopper | 4:30.0h | |
| 13 | Schuylkill Valley | A | Yobb | Kalinowski | Huntsinger | Falsone | 4:30.60 | Daniel Boone @ Schulkill Valley | 
| 14 | Berks Catholic | A | Arentz | Buerke | Sesay | Harakel | 4:40.00 | |
| 15 | Northeastern | A | Wertz | Blaker | Treglia | Coleman | 4:42.71 | |
| 16 | Saint Marys Area | A | Assalone | Bauer | Frontz | Beck | 4:50.24 |