| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | PSU-Harrisburg |
A | Dhital | Samuels | Cavanaugh | Horner | 4:37.98 | United East Indoor Track & Field Championships |
| 3 | Shippensburg |
B | Calamia | Pyle | Malcolm | Wert | 4:15.00 | |
| 4 | Lock Haven |
A | Bair | Halasz | Hauser | Gordon | 4:00.25 | Bison Outdoor Classic 2025 |
| 5 | Juniata |
A | Christophel | McRoberts | Bryant | Reilley | 4:11.77 | Landmark Conference Indoor Track & Field Championships |
| 6 | Shippensburg |
A | Kapschull | Drager | DiCriscio | Logiovine | 4:28.00 | |
| 7 | Georgian Court |
A | Burns | Beasley | Lonseth | Tuttle | DNS |