| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Lock Haven |
A | Chipeleski | Epitropakis | Inch | Wright | 43.88 | |
| 3 | McDaniel |
A | Hubbard | Coleman | Homer | Adelaja | 43.01 | |
| 4 | Shippensburg |
A | Bell III | Galloway | Jackson | Lewis | DNS | 2025 Colonial Relays |
| 5 | Bloomsburg |
A | Hoffman | Leitzel | Umbenhaur | Moore | DNS | |
| 6 | PSU-Harrisburg |
A | Gray | Sodmont | Bogovic | Cammauf | 43.11 | 43.11 Mason-Dixon Track %26 Field Invitational |
| 7 | Lock Haven |
B | Young | Streator | Taylor | Hertzog | DQ |