| MR | Stan Morgan | 48.94 | Duffy, Clippinger, Nickerson, Dukes | Carlisle | 2015 |
| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | |
|---|---|---|---|---|---|---|---|---|
| 1 | Central Dauphin East | A | Baltimore | Livingston | Premici | Turner | 48.48 | Verified |
| 2 | Chambersburg | A | Frazer | Collins | Hang | Holmes | 50.27 | Verified |
| 3 | Waynesboro | A | Anderson | Peyton | French | Creager | 50.32 | Unverified: vs GA |
| 4 | Palmyra | A | Baker | Hipple | Waldron | Miller | 50.93 | Verified |
| 5 | Boiling Springs | A | Hughes | Rauhut | Pabis | Kimmel | 51.97 | Verified |
| 6 | Trinity 03 | A | 52.00 | Verified | ||||
| 7 | Carlisle | A | 53.19 | Unverified: | ||||
| 8 | Cumberland Valley | A | Benini | Garcia | Abdalla | Setuagbe | 53.24 | Unverified: |
| 9 | Mechanicsburg Area | A | 53.54 | Verified |