| MR | Stan Morgan | 3:20.55 | Cumberland Valley | 5/8/2021 | 
| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | |
|---|---|---|---|---|---|---|---|---|
| 1 | Carlisle | A | 3:24.89 | Verified | ||||
| 2 | Cumberland Valley | A | Himmad | Kosofsky | Boland | Grunden | 3:30.24 | Unverified: | 
| 3 | Gettysburg Area | A | Herr | Cole | Nasiri | Clapsadle | 3:31.74 | Unverified: | 
| 4 | Cedar Cliff | A | Schriver | Delevan | Korch | Crawford | 3:34.36 | Verified | 
| 5 | Chambersburg | A | Frey-Reyes | Casagrandi | Harrison II | Shrader | 3:35.60 | Verified | 
| 6 | Mechanicsburg Area | A | 3:37.74 | Verified | ||||
| 7 | Waynesboro | A | Brook | Mencia | Atkinson | Calo | 3:37.74 | Unverified: vs GA | 
| 8 | Lower Dauphin | A | Everett | Frank | Henry | Persing | 3:38.57 | Verified | 
| 9 | Red Land | A | Jackson | Schneider | Lonkart | Walter | 3:41.96 | Verified | 
| 10 | Central Dauphin East | A | Brandenburg | Hake | Brandenburg | Tholey | 3:42.44 | Verified | 
| 11 | Boiling Springs | A | Becker | Verenna | Purdy | Kimmel | 3:43.07 | Verified | 
| 12 | Trinity 03 | A | 3:46.70 | Verified |