| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Kutztown |
A | 3:25.80 | |||||
| 2 | Pitt.-Johnstown |
B | Bertrand | Patterson | Brassart | Weber | 3:20.33 | TFRRS 04/21/23 - 3:45.00 Seed Time |
| 3 | Shippensburg |
A | Bigham | Hrabosky | Kearney | Kirk | 3:15.48 | PSAC Outdoor Track & Field Championships |
| 4 | Indiana (Pa.) |
B | Eberly | Pegher | McGinn | Pacyna | 3:14.72 | TFRRS 04/14/23 - B team. Should be seeded at 3:32 |
| 5 | Indiana (Pa.) |
A | Putt | Stuck | Egizio | Branan | 3:14.72 | Bison Outdoor Classic |
| 6 | Pitt.-Johnstown |
A | Thornton | Lauper | Blum | Lee | 3:20.33 | Mountaineer Showcase |
| 7 | Dickinson |
A | Collins | Penny | Feldman | Lozano | 3:24.05 | TFRRS 05/05/23 - 3:35.00 |
| 8 | Messiah |
A | Provan | Kimmel | Pryor | Kopp | 3:25.92 | TFRRS 05/04/23 - 3:38 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Delaware TC | B | Isacson | Krummenacker | Hutchinson | Carreno | 3:43.13 | Happy Valley Invitational |
| 2 | Penn State T&F Club | C | Sakal | Hallock | Echewa | Christou | 3:28.38 | TFRRS 02/02/24 - 3:40 |
| 3 | Penn State T&F Club | B | Sigler | Reese | Myers | Tomaine | 3:28.38 | TFRRS 02/02/24 - 3:35 |
| 4 | York (Pa.) |
A | Higgins | Apsley | Connolly | Smoot | 3:27.09 | Paul Kaiser Classic |
| 5 | Alvernia |
A | Hahn | Wendel | Dennison | Darlington | 3:27.17 | Dr. Jack M. Toms Alumni Invitational |
| 6 | Penn State T&F Club | A | Castrillon Gonzales | Dupont | Pinheiro | Mambo | 3:28.38 | 2024 YSU Indoor Mid Major |
| 7 | PSU-Harrisburg |
A | Aidel | Gray | Morales | Sodmont | 3:36.99 | United East Indoor Track & Field Championships |
| 8 | Delaware TC | A | Lazev | Wasserman | Wheeler | Kelly | 3:43.13 | TFRRS 02/11/23 - 3:33 |