| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | A | 4:20.42 | 4:20.42 Penn Relays | |
| 3 | Alvernia |
A | 4:18.14 | 4:18.14 19th Annual Paul Kaiser Classic |
| 4 | Misericordia |
A | DNS | 4:16.80 Misericordia Twilight 2024 |
| 5 | York (Pa.) |
A | 4:17.52 | 4:17.52 19th Annual Paul Kaiser Classic |
| 6 | DeSales |
A | 4:20.30 | 4:20.30 Paul Donahue Invitational at Widener University |
| 7 | A | 4:24.23 | 4:24.23 2024 Coach P Invite | |
| 8 | Hood |
A | 4:40.25 | 4:40.25 Hopkins Loyola Invitational |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Delaware Valley |
A | 4:15.24 | 4:15.24 WCU Tune Up |
| 2 | Eastern |
A | 4:13.03 | 4:13.03 Hopkins Loyola Invitational |
| 3 | Albright |
A | 4:10.56 | 4:10.56 Shippensburg University Mid-Week PR Bonanza Meet |
| 4 | Stevens |
A | 4:00.96 | 4:00.96 Penn Relays |
| 5 | Lebanon Valley |
A | 4:09.85 | 4:09.85 2024 Messiah Multi/Invitational |
| 6 | Widener |
A | 4:11.58 | 4:11.58 2024 TCNJ Invite and Multi |
| 7 | Messiah |
A | 4:15.05 | 4:15.05 2024 Messiah Multi/Invitational |
| 8 | A | 4:16.11 | 4:16.11 King%27s College Monarch Open |