| Lane | Team | Squad | Seed |
|---|---|---|---|
| 1 | A | 43.37 | |
| 2 | A | 42.87 | |
| 3 | Alvernia |
A | DQ |
| 4 | DeSales |
A | 41.93 |
| 5 | Misericordia |
A | 42.09 |
| 6 | Stevens |
A | 42.78 |
| 7 | Lebanon Valley |
A | 43.27 |
| 8 | York (Pa.) |
A | 43.39 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Albright |
A | 45.45 | 45.45 2024 Messiah Multi/Invitational |
| 3 | Misericordia |
A | 42.86 | 42.86 Misericordia Invitational 2024 |
| 4 | Widener |
A | DQ | 43.08 Paul Donahue Invitational at Widener University |
| 5 | A | 44.17 | 44.17 Osprey Open | |
| 6 | York (Pa.) |
A | 44.02 | 44.02 Shippensburg University Mid-Week PR Bonanza Meet |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | A | 44.48 | 44.48 Misericordia Invitational 2024 | |
| 3 | Delaware Valley |
A | 44.18 | 44.18 Paul Donahue Invitational at Widener University |
| 4 | Lebanon Valley |
A | 43.72 | 43.72 York College Twilight Meet |
| 5 | A | 43.42 | 43.42 2024 Danny Curran Invitational | |
| 6 | Alvernia |
A | 42.64 | 42.64 Paul Donahue Invitational at Widener University |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Stevenson |
A | 44.44 | 44.44 2024 Towson Invitational |
| 3 | Stevens |
A | 43.62 | 43.62 2024 TCNJ Invite and Multi |
| 4 | Eastern |
A | 43.67 | 43.67 Hopkins Loyola Invitational |
| 5 | DeSales |
A | 41.92 | 41.92 Paul Donahue Invitational at Widener University |
| 6 | Messiah |
A | 44.37 | 44.37 2024 Messiah Multi/Invitational |