| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KARGBO, Aminata | SO | Albright |
NT | |
| 2 | ALYSSA, Devita | JR | Albright |
NT | |
| 3 | TIGHE, Eren | JR | Albright |
NT | |
| 4 | BUTCHER, Ava | SO | 1:02.38 | 2024 Middle Atlantic Conference Outdoor T&F Championships | |
| 5 | VOGEL, Isabel | JR | Stevens |
1:02.00 | |
| 6 | MUHAMMAD EL, Kenya | SO | Albright |
NT |