| Athlete | Yr. | Team | Seed | Note | |
|---|---|---|---|---|---|
| 1 | MALLAST, Blake | JR | Chambersburg | 22.32 | Verified |
| 2 | BESECKER, Kason | SO | Chambersburg | 22.44 | Verified |
| 3 | HARRISON II, Antonio | JR | Chambersburg | 22.52 | Verified |
| 4 | GREEN, Ke'Aune | JR | Cumberland Valley | 23.33 | Verified |
| 5 | HENNES, Hewan | SO | Chambersburg | 23.33 | Verified |
| 6 | COLONNA, Jackson | JR | Cumberland Valley | 23.38 | Verified |
| 7 | EFETURI, Rukie | JR | Cumberland Valley | 23.54 | Verified |
| 8 | ETTERS, Colin | SO | Altoona Area | 23.77 | Verified |
| 9 | STIER, Matthew | SR | Cumberland Valley | 23.78 | Verified |
| 10 | BRAMBLETT, Owen | SO | Cumberland Valley | 24.00 | Unverified: |
| 11 | HIMMAD, Mohamed | JR | Cumberland Valley | 24.03 | Verified |
| 12 | HAZELWOOD, Julian | SO | Altoona Area | 24.13 | Verified |
| 13 | KOSOFSKY, Owen | SO | Cumberland Valley | 24.43 | Verified |
| 14 | FOLTZ, Seth | SO | Cumberland Valley | 24.44 | Verified |
| 15 | TREECE, Josiah | SR | Altoona Area | 24.60 | Verified |
| 16 | EKEZIE, Ogbonnaya | SO | Cumberland Valley | 24.64 | Verified |
| 17 | SHEARER, Keegan | SR | Cumberland Valley | 24.88 | Verified |
| 18 | CHISOLM, Michael | SO | Altoona Area | 24.90 | Unverified: |
| 19 | LAYTON, Alec | 9 | Altoona Area | 24.90 | Unverified: |
| 20 | BAKER, Jahrell | SO | Altoona Area | 25.06 | Verified |
| 21 | ROMANOWICZ, CJ | JR | Altoona Area | 25.16 | Verified |
| 22 | FERTENBAUGH, Dylan | SO | Cumberland Valley | 25.50 | Unverified: |
| 23 | STONER, Noah | JR | Cumberland Valley | 25.75 | Verified |
| 24 | FORD, Gabe | 9 | Cumberland Valley | 26.50 | Unverified: |
| 25 | FOUGHT, Mason | 9 | Cumberland Valley | 26.50 | Unverified: |
| 26 | SHAW, Garrett | JR | Cumberland Valley | 26.60 | Unverified: |
| 27 | WHITEHEAD, Braden | JR | Cumberland Valley | 27.00 | Unverified: |
| 28 | WILLIAMS, Luke | 9 | Cumberland Valley | NT | Unverified: |
| 29 | HIMMAD, Ayoub | SO | Cumberland Valley | NT | Unverified: |