| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | |
|---|---|---|---|---|---|---|---|---|
| 1 | JP McCaskey | A | 48.44 | Unverified: | ||||
| 2 | Warwick | A | 49.82 | Verified | ||||
| 3 | Conestoga Valley | A | Linder | Denlinger | Koenig | Montijo-Ortiz | 50.04 | Unverified: |
| 4 | Exeter Township | A | Amouzou | Miller | Ware | Nerney | 51.63 | Unverified: |
| 5 | Cedar Crest | A | Noecker | Pavlesich | Hoffman | Ojo | 51.74 | Unverified: |
| 6 | Governor Mifflin | A | 51.74 | Unverified: | ||||
| 7 | Penn Manor | A | Washington | Phillips | Harnish | Lee | 51.81 | Verified |
| 8 | Garden Spot | A | 52.34 | Unverified: | ||||
| 9 | Avon Grove | A | Scott | El-Attar | Hopkins | Kurtz | 52.63 | Verified |
| 10 | Northeastern | A | Calder | Jackson | Metzler | Owens | 52.75 | Verified |
| 11 | West York Area | A | 53.12 | Verified | ||||
| 12 | Hempfield 03 | A | Givler | Allgyer | Sweigart | Olivencia | 53.45 | Unverified: |
| 13 | Lampeter Strasburg | A | Herr | Jannone | Holland | Ongosi | 53.74 | Unverified: LS Dual |
| 14 | Ephrata | A | Schwartz | Sakyiwaa - Yeboah | Faccio | Harris | 54.04 | Unverified: |
| 15 | Lebanon | A | Burrus | Rivera Colon | Whigham | Whigham | 54.85 | Verified |
| 16 | Schuylkill Valley | A | Falsone | Sensenig | Yobb | Stork | 55.32 | Verified |
| 17 | Lancaster Catholic | A | McCuen | Cintron | McFadden | Walker | 55.83 | Verified |