| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | |
|---|---|---|---|---|---|---|---|---|
| 1 | Ephrata | A | Knowles | Collazo-Colon | Loose | Keller | 3:26.81 | Unverified: |
| 2 | JP McCaskey | A | 3:32.24 | Unverified: | ||||
| 3 | Cedar Crest | A | Williams | Joffy | Fretz | Ruth | 3:34.94 | Unverified: Cedar Crest vs Hempfield |
| 4 | Conestoga Valley | A | Jackson | Miller | Gitonga | Castronova | 3:35.06 | Verified |
| 5 | Exeter Township | A | Morgan | Nester | Boyd | Petersen | 3:37.10 | Unverified: |
| 6 | Solanco | A | Wright | Lenhart | Lenhart | Bangura-Ketima | 3:37.24 | Unverified: |
| 7 | Penn Manor | A | Menaquale | Weaver | Hightower | Kabakjian | 3:37.74 | Unverified: Manheim Township HS vs Penn Mano |
| 8 | Daniel Boone Area | A | 3:38.70 | Unverified: | ||||
| 9 | Garden Spot | A | 3:39.00 | Unverified: | ||||
| 10 | Warwick | A | 3:39.97 | Verified | ||||
| 11 | Lebanon | A | Long | Kreider | Rivera | Itzen | 3:40.88 | Verified |
| 12 | West York Area | A | 3:46.24 | Unverified: | ||||
| 13 | Governor Mifflin | A | 3:47.56 | Verified | ||||
| 14 | Manheim Central | A | Kenneff | Lubeskie | Moffett | Sargent | 3:49.24 | Unverified: |
| 15 | Schuylkill Valley | A | Maciejewski | Wood | Bechtel | Sabo | 3:52.24 | Unverified: |
| 16 | Lancaster Catholic | A | Hummer | Montalvo-Melecio | Hollenbacher | Clemson | 3:54.19 | Verified |
| 17 | Northeastern | A | Gorkoyah | Richard | Ridler | Galloway | 3:54.73 | Verified |