| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Dickinson |
A | Naqvi | Kurey | Kratovil-Lavelle | Priebe | 4:20.00 | |
| 3 | Shippensburg |
A | Drager | DiCriscio | Whysong | Wilson | 4:16.00 | |
| 4 | East Stroudsburg |
A | Domurat | Smith | Roth | Cancel | 4:10.00 | |
| 5 | Albright |
A | Callahan | Tighe | Dover | Gingrich | 4:15.00 | Ursinus 4/5/24 |
| 6 | Lock Haven |
A | Fite | Gearinger | Gentzel | Sechrist | DNS | |
| 7 | Albright |
B | Schweitzer | Parmelee | Brown | Devita | DNF |