| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lock Haven |
C | Chipeleski | Epitropakis | Inch | Smith | 44.43 | |
| 2 | York (Pa.) |
A | Hardison | Niemandt | Friend | Higgins | 43.58 | Shippensburg University College Mid-Week Meet |
| 3 | Lock Haven |
B | Cassetori | Capparelli | Wright | Miller | 42.78 | |
| 4 | Lock Haven |
A | Whitaker | Lawrence | Kravetz | Shoats | 42.05 | 2023 LHU Qualifier |
| 5 | Juniata |
A | Riddle | Lewis | Massare | Huffstutler | 42.50 | |
| 6 | Bloomsburg |
A | Cronan | Wilson | Purtle | Walsh | 43.11 | Mid Week Quad-Meet (Lock Haven) |
| 7 | Albright |
A | Villano | Hammond | Maley | Saydowary | 43.73 | 2023 Widener Invite |
| 8 | Lock Haven |
D | Watson | Streator | Young | Rouse | 44.98 |