| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Delone Catholic | A | 3:53.0h | |||||
| 2 | Bermudian Springs | A | Carrolus | Harner | Kehr | Stuart | 3:41.32 | Bruce Dallas Invitational |
| 3 | Gettysburg Area | A | Herr | Cole | Nasiri | Clapsadle | 3:31.5h | |
| 4 | Fairfield Area | A | Herb | Davis | Davis | Brown | 3:37.91 | |
| 5 | Littlestown | A | Bittle | Clabaugh | Kabrick | Miller | DNS | Hanover |