| Athlete | Yr. | Team | Seed | Note | |
|---|---|---|---|---|---|
| 1 | CAMPBELL, Samantha | FR | Gettysburg Area | 5:37.7h | |
| 2 | ARNOLD, Lily | JR | Gettysburg Area | 5:45.5h | |
| 3 | LIGHTY, Livia | JR | Bermudian Springs | 6:02.25 | |
| 4 | KAHN, Mirena | FR | Gettysburg Area | 6:28.8h | |
| 5 | MAUSS, Isabel | SO | Biglerville | 6:34.9h | Biglerville v Bermudian 2024 |
| 6 | MAUSS, Grace | FR | Biglerville | 6:44.0h | Biglerville v Farifield 2024 |
| 7 | MYERS, Abigail | FR | Bermudian Springs | 6:45.71 | Arctic Blast Invitational |
| 8 | KNOX, Olivia | FR | Delone Catholic | 6:55.50 | The Miller Sports Construction B |