| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Lock Haven |
B | 43.22 | |
| 3 | Georgian Court |
A | 42.55 | Shippensburg University College Mid-Week Meet |
| 4 | Hood |
A | 42.00 | |
| 5 | Lock Haven |
A | 42.21 | |
| 6 | York (Pa.) |
A | 42.89 | 17th Annual Paul Kaiser Classic |
| 7 | Lock Haven |
C | 46.18 |