| Athlete | Yr. | Team | Seed | Note | |
|---|---|---|---|---|---|
| 1 | BEST, Roderick | SR | Musketeers Track & Field Club | 53.00 | |
| 2 | WHITFIELD, Sean | JR | Goucher |
53.00 | |
| 3 | HONEYBLUE, Mustafa | JR | Goucher |
53.00 | |
| 4 | DI CESARE, Alec | SO | Lebanon Valley |
53.00 | |
| 5 | BRUCHAK, Matthew | SO | Lebanon Valley |
53.50 | |
| 6 | LEWIS, Deonte | FR | Goucher |
54.00 | |
| 7 | MCKELL, Elijah | FR | Lebanon Valley |
54.00 | |
| 8 | GOUNDIE, Donovan | FR | Lebanon Valley |
54.00 | |
| 9 | FERRIZZI, Vince | JR | Lebanon Valley |
54.00 | |
| 10 | RESELE, Nicholas | SO | Lebanon Valley |
56.00 | |
| 11 | VELLIOS, Gabriel | FR | Lebanon Valley |
56.00 | |
| 12 | HARTIGAN, Richard | SO | 56.56 | Blue Jay Tune-Up | |
| 13 | ROTHSTEIN, Samuel | FR | Lebanon Valley |
57.00 | |
| 14 | LOY, Andrew | JR | Lebanon Valley |
57.00 | |
| 15 | ESCHINGER, McKendry | JR | Lebanon Valley |
58.00 | |
| 16 | MORRISON, Christian | FR | Lebanon Valley |
58.00 | |
| 17 | SCARBOROUGH, Caleb | SO | Lebanon Valley |
58.00 | |
| 18 | LEHMAN, Cole | SO | Lebanon Valley |
58.00 | |
| 19 | BENEDICT, Joey | FR | 58.00 | ||
| 20 | RODOFF, Darren | JR | Lebanon Valley |
59.00 |