| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | COLONNA, Jackson | SO | Cumberland Valley | 23.59 | Verified | 
| 2 | MILES, Trevor | SO | Mifflin County High School | 25.87 | Verified | 
| 3 | FREDERICK, Nate | SR | Cumberland Valley | 23.04 | Verified | 
| 4 | KRATZER, Jacob | JR | Mifflin County High School | 25.55 | Verified | 
| 5 | SODMONT, Kadin | SR | Cumberland Valley | 23.40 | Unverified: | 
| 6 | SIMMONS, Myka | JR | Mifflin County High School | NT | Unverified: | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | STIER, Matthew | JR | Cumberland Valley | 23.94 | Verified | 
| 2 | SMITH, Carter | 9 | Mifflin County High School | NT | Unverified: | 
| 3 | WILMARTH, Colin | JR | Cumberland Valley | 23.84 | Verified | 
| 4 | GARLAND, Gideon | 9 | Mifflin County High School | NT | Unverified: | 
| 5 | SINGH, Kern | SO | Cumberland Valley | 23.92 | Verified | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | BRAMBLETT, Owen | 9 | Cumberland Valley | 24.93 | Verified | 
| 2 | HIMMAD, Mohamed | SO | Cumberland Valley | 24.45 | Verified | 
| 3 | CHESNEY, Pierce | SR | Cumberland Valley | 24.03 | Verified | 
| 4 | KOSOFSKY, Owen | 9 | Cumberland Valley | 24.43 | Verified | 
| 5 | GREEN, Keaune | SO | Cumberland Valley | 24.50 | Unverified: | 
| 6 | EFETURI, Rukie | SO | Cumberland Valley | 24.99 | Verified | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | STONER, Noah | SO | Cumberland Valley | 25.89 | Verified | 
| 2 | SHEARER, Keegan | JR | Cumberland Valley | 25.58 | Verified | 
| 3 | FOLTZ, Seth | 9 | Cumberland Valley | 25.08 | Verified | 
| 4 | SHUGHART, Greyson | SR | Cumberland Valley | 25.40 | Unverified: | 
| 5 | EKEZIE, Ogbonnaya | 9 | Cumberland Valley | 25.63 | Verified | 
| 6 | BEY JR, Hadiel | SO | Cumberland Valley | 26.37 | Verified | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 2 | THAMPY, Pranav | 9 | Cumberland Valley | NT | Unverified: | 
| 3 | DIXSON, Jack | 9 | Cumberland Valley | 27.20 | Unverified: | 
| 4 | SIDDIQUI, Ahmed | JR | Cumberland Valley | NT | Unverified: |