| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | Catholic |
A | DNS | Diplomat Open @ Franklin & Marshall |
| 4 | Bloomsburg |
A | 3:27.49 | 23rd Annual Bison Open |
| 5 | Shippensburg |
A | 3:27.00 | |
| 6 | Rowan |
A | DQ |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | Lebanon Valley |
A | 3:41.00 | |
| 4 | Bloomsburg |
B | 3:40.00 | |
| 5 | Stevens |
A | 3:36.00 | |
| 6 | York (Pa.) |
A | 3:40.00 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | A | 3:56.39 | 2021 Golden Wolves Rust Buster | |
| 4 | Misericordia |
A | 3:45.00 | |
| 5 | Delaware Valley |
A | 3:41.64 | 2021 Golden Wolves Rust Buster |
| 6 | York (Pa.) |
B | 3:50.00 |