| Team | Squad | Seed | Note | |
|---|---|---|---|---|
| 1 | Shippensburg |
A | 3:27.00 | |
| 2 | Bloomsburg |
A | 3:27.49 | 23rd Annual Bison Open |
| 3 | Rowan |
A | 3:30.17 | |
| 4 | Catholic |
A | 3:30.67 | Diplomat Open @ Franklin & Marshall |
| 5 | Stevens |
A | 3:36.00 | |
| 6 | Bloomsburg |
B | 3:40.00 | |
| 7 | York (Pa.) |
A | 3:40.00 | |
| 8 | Lebanon Valley |
A | 3:41.00 | |
| 9 | Delaware Valley |
A | 3:41.64 | 2021 Golden Wolves Rust Buster |
| 10 | Misericordia |
A | 3:45.00 | |
| 11 | York (Pa.) |
B | 3:50.00 | |
| 12 | A | 3:56.39 | 2021 Golden Wolves Rust Buster |