| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Milton Hershey | A | 4:00.01 | Unverified: Tim Cook Memorial Invitational |
| 2 | Trinity (3) | A | 3:40.98 | Verified |
| 3 | Shamokin Area | A | 3:58.46 | Unverified: Breslin Invitational at Mount Ca |
| 4 | Susquenita | A | 3:37.82 | Verified |
| 5 | Cedar Cliff | A | 3:49.00 | Unverified: |
| 6 | Lower Dauphin | A | 4:15.00 | Unverified: Shippensburg 95th Roddick HS Inv |