| R | Bruce Dallas T&F Invitational | 4:01.83 | Cumberland Valley | 4/3/2021 |
| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | |
|---|---|---|---|---|---|---|---|---|
| 1 | Cumberland Valley | A | Heilman | Pines | Boardman | Ebersole | 3:56.50 | Verified |
| 2 | Wilson (3) | A | Arentz | Brown | Culp | Dallas | 4:09.70 | Verified |
| 3 | Lewisburg Area | A | Brazier | Ikeler | Blakeslee | Malone | 4:10.00 | Unverified: |
| 4 | Manheim Township | A | Ebersole | Weiss | Fogleman | Papadimitriou | 4:21.40 | Unverified: |
| 5 | Lower Dauphin | A | Bretz | Gula | Hollenbach | Swartz | 4:23.15 | Verified |
| 6 | Carlisle | A | Leatherman | Podzic | Cessna | Ring | 4:24.00 | Unverified: |
| 7 | Northeastern | A | Reihm | Brown | Wertz | Calder | 4:26.30 | Unverified: |
| 8 | Phoenixville Area | A | Barbash | Carruthers | Carr | Reed | 4:29.37 | Verified |
| 9 | Milton Hershey | A | Tejada | Konneh | Washington | McClellan | 4:29.40 | Verified |
| 10 | Palmyra | A | Chapin | Hudson | McConaghy | Newman | 4:30.00 | Unverified: |
| 11 | Conestoga Valley | A | Santamaria | Cintron | Frankford | Lapp | 4:35.00 | Unverified: |
| 12 | Cedar Cliff | A | LaFountain | Joy | Faircloth | Nugin | 4:36.52 | Verified |
| 13 | Susquehanna Township | A | Weidler | Greene | Whitehead | Dorsey | 4:40.00 | Unverified: |
| 14 | Minersville Area | A | Burgess | Corley | Donnelly | Grimm | 4:40.00 | Unverified: |
| 15 | Spring Grove | A | Gerber | Wilson | Kintner | Houghton | 4:50.00 | Unverified: |
| 16 | Harrisburg High School | A | Peterson-Chambers | Rankin | Porter | Greene | 4:50.00 | Unverified: |