| Athlete | Yr. | Team | Seed | Note | |
|---|---|---|---|---|---|
| 1 | WRIGHT, Kamiah | SR | J.P. McCaskey | 59.39 | Verified |
| 2 | HELDERMAN, Marissa | JR | Warwick | 59.40 | Verified |
| 3 | MILLER, Sarah Ann | SR | Twin Valley | 1:02.12 | Verified |
| 4 | CARRINGTON, Lanae | JR | Harry S Truman | 1:02.37 | Verified |
| 5 | LEA, Alexandra | JR | Southern Lehigh | 1:02.50 | Unverified: |
| 6 | PRATT, Addison | SO | Twin Valley | 1:03.20 | Verified |
| 7 | BARD, Sarah | 9 | Solanco | 1:04.00 | Unverified: |
| 8 | HAKES, Allyson | JR | Pottsgrove | 1:04.00 | Unverified: |
| 9 | BROWN, Amani | SR | Northeastern | 1:05.73 | Verified |
| 10 | LEWIS, Anya | SO | Central Dauphin | 1:06.24 | Unverified: |
| 11 | PROCTOR, Rianna | SO | Central Dauphin | 1:06.48 | Unverified: |
| 12 | BUTTON, Kennedy | JR | Central Bucks East | 1:06.78 | Verified |
| 13 | GLENN, Alleha | SO | Warwick | 1:07.00 | Unverified: |
| 14 | NEISWENDER, Sarina | SO | Conrad Weiser | 1:07.22 | Unverified: CW Trials |
| 15 | ODESSEY, Ciana | SO | Louis E. Dieruff | 1:08.00 | Unverified: |
| 16 | BLAKER, Sierra | SO | Northeastern | 1:08.30 | Verified |
| 17 | ESTRELLA, Jasmine | JR | Octorara | 1:09.00 | Unverified: |
| 18 | LAWSON, Amaya | SO | Central Dauphin | 1:09.24 | Unverified: |
| 19 | GOTAY MOREIRA, Nisani | SO | Governor Mifflin | 1:09.71 | Verified |
| 20 | YAZDANYAR, Elizabeth | SO | Southern Lehigh | 1:09.90 | Verified |
| 21 | POWL, Emily | SO | Solanco | 1:10.00 | Unverified: |
| 22 | HAIRSTON, Rebecca | JR | Dallastown | 1:10.00 | Unverified: |
| 23 | LAUER, Lexi | JR | Central Dauphin | 1:10.24 | Unverified: |
| 24 | ASHFIELD, Emma | JR | Marian Catholic | 1:11.20 | Unverified: |
| 25 | MCFARLAND, Kelsey | SR | Harry S Truman | 1:11.40 | Verified |
| 26 | PIKITUS, Alizabeth | SO | Marian Catholic | 1:12.20 | Unverified: |
| 27 | HARRISTON, Keyara | JR | William Allen | 1:12.68 | Verified |
| 28 | GROFF, Addison | SO | Solanco | 1:12.74 | Verified |
| 29 | DICESARE, Tessa | SO | Southern Lehigh | 1:12.84 | Verified |
| 30 | HIGHDUCH, Kaitlyn | JR | Governor Mifflin | 1:13.00 | Unverified: |
| 31 | BRAY, Erin | SO | Cardinal O'Hara | 1:13.00 | Unverified: |
| 32 | WIKER, Kenzee | SO | Warwick | 1:13.00 | Unverified: |
| 33 | BISSINGER, Madelyn | SO | Cardinal O'Hara | 1:13.00 | Unverified: |
| 34 | KIERNAN, Ella | SO | Central Bucks East | 1:13.00 | Unverified: |
| 35 | SMITH, Saige | 9 | Southern Lehigh | 1:13.00 | Unverified: |
| 36 | FRITZ, Ava | SO | Marian Catholic | 1:13.10 | Unverified: |
| 37 | SHABAZZ SCOTT, Nyiera | SO | West York Area | 1:13.22 | Verified |
| 38 | HEDGCOCK, Alyssa | SO | West York Area | 1:13.38 | Verified |
| 39 | ROTONDO, Isabella | SO | Central Bucks East | 1:14.00 | Unverified: |
| 40 | DRAGO, Lydia | SO | Governor Mifflin | 1:15.00 | Unverified: |
| 41 | VENTURA, Arianna | SO | Fleetwood Area | 1:15.52 | Verified |
| 42 | DRAGO, Faith | SR | Governor Mifflin | 1:17.00 | Unverified: |
| 43 | DEMPSEY, Kayleigh | 9 | Cardinal O'Hara | 1:18.00 | Verified |
| 44 | SURACE, Keira | 9 | Cardinal O'Hara | 1:18.00 | Unverified: |
| 45 | STEVENS, Sade | 9 | Cardinal O'Hara | 1:20.00 | Unverified: |
| 46 | SMITH, Luci | SO | Phoenixville Area | 1:20.00 | Unverified: |
| 47 | RIES, Amanda | 9 | Central Bucks East | 1:20.00 | Unverified: |
| 48 | SOMOYE, Ikram | 9 | Harry S Truman | 1:20.24 | Unverified: |
| 49 | WRATTO, Naomi | 9 | Harry S Truman | 1:20.24 | Unverified: |
| 50 | REILLEY, Chloe | JR | Twin Valley | 1:22.03 | Verified |
| 51 | LEE, Maria | JR | Cardinal O'Hara | 1:23.00 | Unverified: |
| 52 | STEITZ, Elizabeth | 9 | Central Bucks East | 1:25.00 | Unverified: |
| 53 | SANCHEZ, Rosnelly | SR | Reading | 1:26.00 | Unverified: |
| 54 | TRADER, Zeyvani | 9 | William Allen | NT | Unverified: |
| 55 | AUGUSTE, Lynn | 9 | William Allen | NT | Unverified: |
| 56 | DOUGLAS, Octavia | 9 | William Allen | NT | Unverified: |
| 57 | GARCIA, Ashly | 9 | William Allen | NT | Unverified: |