| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Alvernia |
A | Loehr | Long | Short | Lawrence | 1:34.20 | Golden Wolves Rust Buster |
| 2 | Susquehanna |
A | Debastiani | Coulter | Gayles | Christian | 1:34.09 | Conference Challenge Cup Invite |
| 3 | Moravian |
A | McFarland | Lutz | Stewart | Porter | 1:34.00 | |
| 4 | Lebanon Valley |
B | Weck | Sheffield | Edmondson | West | 1:33.20 | |
| 5 | Widener |
A | Ayichi | Gould | Ludlow | John | 1:32.11 | |
| 6 | Lebanon Valley |
A | Grauer | Reed | Smith | Buhr | 1:33.20 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | A | Erisman | Klutinoty | Noll | Zimmerman | NT | ||
| 2 | Widener |
B | Gremban | Savela | Davis | Harris | DNS | |
| 3 | Albright |
A | Harris | Camara | Belfield | Negron | 1:38.34 | Coach I Open |
| 4 | Eastern |
A | Danglade | Koehler | Moore | Andre | 1:35.52 | Coach I Open |
| 5 | Catholic |
A | Novak | Dreitlein | Beckett | Maxwell | 1:35.00 | |
| 6 | Elizabethtown |
A | Niles | Mann | Belgrave | Schwab | 1:35.58 | Orange and Maroon Classic and Multi |