| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | STOUT, Austin | Virginia Tech Running Club | 50.50 | ||
| 2 | KELLY, Cody | SR | Seton Hill | 50.00 | |
| 3 | BOEA-GISLER, Avery | SO | California (Pa.) | 49.50 | Bison Outdoor Classic |
| 4 | HARRIS, Dalon | FR | CCBC Catonsville | 47.40 | |
| 5 | TINDAL, T.J. | SO | Wheeling Jesuit | 48.80 | |
| 6 | FRAZIER, Orion | FR | West Chester | 49.75 | |
| 7 | CASTELLO, Ricky | SR | Cortland St. | 50.40 | |
| 8 | SILVESTRE, Eliezier | SO | Indiana (Pa.) | 50.50 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BEVILAQUA, Joe | FR | West Chester | 50.90 | |
| 2 | MCCASKILL, Marcel | SR | Wheeling Jesuit | 50.80 | |
| 3 | CWIKLINSKI, Thaddeus | SR | Gettysburg | 50.66 | Little 3 Championship |
| 4 | KASHMER, Zachary | JR | Cortland St. | 50.60 | |
| 5 | CAMPBELL, Kohl | SO | Wheeling Jesuit | 50.66 | Duke Invitational |
| 6 | UPDEGRAFF, Luke | FR | Cortland St. | 50.75 | |
| 7 | EUBANKS, Michael | FR | Cortland St. | 50.85 | |
| 8 | REID, Steven | JR | Indiana (Pa.) | 51.00 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WELLER, Matthew | FR | Gettysburg | 52.96 | Little 3 Championship |
| 2 | CHARLES, Tyriek | JR | Frostburg State | 52.54 | 2019 Messiah Multi/Invitational |
| 3 | MCELHENY, Theoren | FR | Seton Hill | 51.50 | |
| 4 | CARRIGAN, Peter | FR | York (Pa.) | 51.00 | |
| 5 | BROOKS, Isaiah | SO | Cortland St. | 51.21 | |
| 6 | VELOZWONG, Anderson | FR | Lebanon Valley | 52.00 | |
| 7 | LOEHR, Adam | FR | Alvernia | 52.88 | |
| 8 | CHANG, Vincent | FR | York (Pa.) | 53.00 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | O'BRIEN, Cael | SO | Gettysburg | 54.43 | Little 3 Championship |
| 2 | JACKSON, Jerome | SO | PSU-Harrisburg | 54.00 | |
| 3 | STEM, Nicholas | SO | York (Pa.) | 53.50 | |
| 4 | BRAUNWORTH, Shane | SR | Messiah | 53.24 | 2019 Messiah Multi/Invitational |
| 5 | ROBERTS, Montray | SR | Gallaudet | 53.49 | Gallaudet vs Medar Evers |
| 6 | HOFFMAN, Cassidy | FR | Messiah | 54.00 | |
| 7 | BROUILLARD, Angus | FR | Gettysburg | 54.23 | Little 3 Championship |
| 8 | HOVIS, Cole | FR | PSU-Harrisburg | 55.00 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ROBINSON, Dmitri | FR | Frostburg State | 56.65 | Frostburg State University Bobcat Track & Field Invitational |
| 2 | HOWE, Elijah | FR | Goldey-Beacom | 56.00 | |
| 3 | TIDAY, Gabriel | FR | Messiah | 55.36 | Shippensburg University Mid-Week Meet |
| 4 | RABSAHL, Sven | SO | Seton Hill | 55.10 | Bison Outdoor Classic |
| 5 | BARNES, James | JR | Gettysburg | 55.31 | 2019 Mason-Dixon Invitational |
| 6 | MCCARTHY, Kyle | Virginia Tech Running Club | 56.00 | ||
| 7 | KOWGIOS, Nicholas | SO | Gettysburg | 56.50 | |
| 8 | FIUCCI, Nicholas | SO | Alvernia | 58.00 |