| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Messiah | A | Wiederrecht, Kathryn | McMinn, Abby | Longshaw, Ellie | Wiederrecht, Taylor | 49.32 | 2019 Messiah Multi/Invitational |
| 2 | California (Pa.) | A | Ross, Innocense | Franklin, Divonne | Lewis, Aaliyah | Collier, Alicia | 47.95 | |
| 3 | West Chester | A | Lewis, Caroline | Hegarty, Amanda | King, Allison | Jackson, Cara | 47.50 | |
| 4 | Shippensburg | A | Wyland, Ali | Williams, Zarria | Jones, Ariel | Ramsden, Mary | 46.89 | PSAC Outdoor Track & Field Championships |
| 5 | Millersville | A | Doherty, Brinn | Reviere, Emonie | Liebl, Kay | Holloman, Destinee | 47.50 | |
| 6 | Cortland St. | A | Gardner, Kelly | Campbell, Maya | Batista, Steffany | Hunter, Taylor | 47.81 | |
| 7 | Wheeling Jesuit | A | Rosenberg, Devyn | McIntosh, Ashley | Massa, Anna | Benjamin, Aniyah | 48.03 | |
| 8 | Johns Hopkins | A | Bothwell, Haley | Nwankpa, Chinaza | Ayuso, Marina | Raymond, Seye | 49.50 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | York (Pa.) | A | Llamas, Nancy | Bria, Kristin | Wagner, Jordan | Winkelspecht, Rylee | 54.00 | |
| 3 | Virginia Tech Running Club | A | Thompson, Lindsey | Bourret, Kira | Messner, Nicole | Colbert, Hannah | 52.00 | |
| 4 | Lebanon Valley | A | Burkhart, Anna | Olewine, Jessica | Ford, Rebecca | Diffenderfer, Alyssa | 50.42 | |
| 5 | Frostburg State | A | Reason, Gabbriell | Estrada, Keileen | Brown, Sidonie | Watson, Maddison | 51.13 | 2019 Mason-Dixon Invitational |
| 6 | Juniata | A | Walshaw-Wertz, Meadow | Gasmen, Caitlyn | Caldwell, Holly | Nichols, Faith | 53.74 |