| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ELLIOT, Alexa | Delaware Valley |
NT | ||
| 2 | YOUNG, Gretchen | Hood |
NT | ||
| 3 | FERNANDEZ CRUZ, Ana | Alvernia |
1:20.00 | ||
| 4 | ORZECHOWSKI, Kailey | 1:06.14 | |||
| 5 | HERZOG, Olivia | Alvernia |
1:03.00 | ||
| 6 | CAMPBELL, Joy | SR | Albright |
1:17.00 |