| Athlete | Yr. | Team | Seed | Note | |
|---|---|---|---|---|---|
| 1 | BOYKIN, Jesikah | SR | Cardinal O'Hara | 1:01.36 | Verified |
| 2 | SCHRAUDNER, Trinity | JR | Northeastern | 1:03.85 | Verified |
| 3 | DALLAS, Katie | SO | Wilson (3) | 1:06.00 | Unverified: |
| 4 | COLLINS, Sabryna | SO | Governor Mifflin | 1:07.00 | Unverified: |
| 5 | GARDNER, Ariel | JR | Governor Mifflin | 1:07.00 | Unverified: |
| 6 | FORREN, Jaden | JR | Solanco | 1:07.00 | Unverified: |
| 7 | SEA, Rory | 9 | Governor Mifflin | 1:08.00 | Unverified: |
| 8 | ULRICH, Rachel | SO | Governor Mifflin | 1:08.00 | Unverified: |
| 9 | ANGSTADT, Emmy | 9 | Governor Mifflin | 1:10.00 | Unverified: |
| 10 | BYERS, Haley | SO | Solanco | 1:10.00 | Unverified: |
| 11 | GRIMM, Imani | SO | Minersville Area | 1:11.00 | Unverified: |
| 12 | SKAYHAN, Julia | SR | Twin Valley | 1:11.11 | Verified |
| 13 | HESS, Kaitlyn | SO | Delaware Military Academy | 1:12.00 | Unverified: |
| 14 | HILL, Olivia | SR | Governor Mifflin | 1:12.00 | Unverified: |
| 15 | CORDOVA, Mary | JR | Wilson (3) | 1:12.00 | Unverified: |
| 16 | MCCOY, Sam | SO | Northeastern | 1:14.00 | Unverified: |
| 17 | GENAO, Isabella | SO | Marian Catholic | 1:15.00 | Unverified: |
| 18 | MOORE, Lauren | 9 | Cardinal O'Hara | 1:16.00 | Unverified: |
| 19 | JONES, Emma | SO | Twin Valley | 1:16.38 | Verified |
| 20 | RENTSCHLER, Audrey | 9 | Hamburg Area | 1:17.00 | Unverified: |
| 21 | FRYBERGER, Paige | 9 | Governor Mifflin | 1:20.00 | Unverified: |
| 22 | SHARP, Kathryn | SO | Twin Valley | 1:22.30 | Verified |