| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Augusta | A | Doyle | Skov | Brown | Jester | 53.42 | AVCTL Division III Championships |
| 2 | Chapman | A | Baer | Harris | Calovich | Wehrmeister | 52.09 | |
| 3 | Clay Center | A | Johnston | Pladson | Craig | Catlin | 51.40 | NCKL |
| 4 | Abilene | A | WELLS | Barbieri | Waite | Heintz | 50.96 | Beloit Relays |
| 5 | Concordia | A | Bowers | Nuss | Hamel | Kueker | 51.31 | Minneapolis V/JV Meet |
| 6 | McPherson | A | Wilson | Buschbom | Dossett | McLeod | 51.49 | AVCTL Division III Championships |
| 7 | El Dorado | A | Nichols | Perrill | Kemboi | Scott | 53.05 | Marion Relays |
| 8 | Buhler | A | Goertz | Lackey | Leving | Heinlein | NT | Salina Central Invite |