| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Note |
|---|---|---|---|---|---|---|---|
| 1 | Pike Valley | A | Benne | Newman | Russell | Sublett | Smith Center Redmen Relays |
| 2 | Republic Co | A | White | Siemsen | Morris | Gaither | Smith Center Redmen Relays |
| 3 | Clifton-Clyde | A | 2025 Lions Club Invite | ||||
| 4 | Axtell | A | Buessing | Broxterman | Schmelzle | Talbot | 2025 Lions Club Invite |
| 5 | Blue Valley | A | Sump | Cox | Irvine | Clark | Falcon Relays |
| 6 | Hanover | A | Meyn | Jueneman | Kickhaefer | Bruna | 2025 Lions Club Invite |
| 7 | Linn | A | Ohlde | Ohlde | Thalmann | Ohlde | 2025 Lions Club Invite |