| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Note |
|---|---|---|---|---|---|---|---|
| 1 | Valley Heights | A | Steinfort | Miller | Porter | McIntyre | 2025 Lions Club Invite |
| 2 | Republic Co | A | Thumann | Hoesli | Clausen-Peters | Smith | Smith Center Redmen Relays |
| 3 | Blue Valley | A | Corkill | Dumler | Brockman | Moses | Falcon Relays |
| 4 | Centralia | A | Kramer | Kramer | Kramer | Tanking | NC Meet |
| 5 | Hanover | A | Martin | Jueneman | Bruna | Heiman | 2025 Lions Club Invite |
| 6 | Washington Co | A | Goeckel | Baker | Hoover | Jueneman | 2025 Lions Club Invite |
| 7 | Clifton-Clyde | A | Osborne |