| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Note |
|---|---|---|---|---|---|---|---|
| 1 | St. John's | A | Holdren | Letourneau | Niewald | Roderick | Smith Center Redmen Relays |
| 2 | Concordia | A | Marsh | Gallardo | Jackson | Barnes | 2025 Abilene HS Varsity Invite |
| 3 | Nickerson | A | Brinkley | Brinkley | Pauley | Huggard | Sterling Relays 2025 |
| 4 | Smith Center | A | Enochs | Hickert | Doane | Schaffer | Smith Center Redmen Relays |
| 5 | Chapman | B | Calovich | Crouse | Solander | Nisbeth | |
| 6 | Smoky Valley | A | Olson | Ayala | Patrick | Bishop |