| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | FOWLER, Serenity | SO | Appalachian Christian School | 44.00 | |
| 4 | DRUMHELLER, Mackenzie | FR | Blue Ridge Christian School | 37.05 | |
| 5 | THOMPSON, Amy | SR | Appalachian Christian School | 39.00 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | MCGHEE, Liliana | 8 | Roanoke Valley Christian | 35.36 | |
| 3 | MILLER, Ada | SO | Redeemer Classical High School | 33.90 | |
| 4 | TRIVETTE, Caroline | FR | Regents School of Charlottesville | 33.31 | |
| 5 | HOBSON, Lamaya | FR | Roanoke Valley Christian | 33.49 | |
| 6 | WOOD, Julia | SO | Appalachian Christian School | 35.00 | |
| 7 | SWARTZ, Victoria | FR | Blue Ridge Christian School | 36.24 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WALLS, Julia | SO | Blue Ridge Christian School | 31.63 | |
| 2 | RUIZ, Marlee-May | FR | Christian Heritage Academy | 31.12 | |
| 3 | MORRIS, Bri | 8 | Roanoke Valley Christian | 29.44 | |
| 4 | WHITE, Julia | FR | Christian Heritage Academy | 28.34 | |
| 5 | ATKINS, Lydia | SR | Christian Heritage Academy | 28.81 | |
| 6 | ARMENTROUT, Olivia | FR | Cornerstone Christian School | 30.71 | |
| 7 | GORDON, Sarah | FR | Blue Ridge Christian School | 31.17 | |
| 8 | HURST, Violet | SO | Blue Ridge Christian School | 31.88 |