| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | PAULEY, Maren | James River (Buchanan) | NT | |
| 2 | MYLES, Ainsley | North Cross | NT | |
| 3 | FADIA, Brookelyn | Lord Botetourt | NT | |
| 4 | SIFUENTES, Krystal | Northside High School | 3:50.78 | Northside Invitational |
| 5 | HARDING, Lilah | Hidden Valley High School | NT | |
| 6 | TULOU, Marie | Patrick Henry (Roanoke) | NT | |
| 7 | MARKHAM, Ariana | Northside High School | NT | |
| 8 | RECKLEY, Elizabeth | Patrick Henry (Roanoke) | NT | |
| 9 | FUQUA, Farrah | Northside High School | NT | |
| 10 | GOUDES, Shelby | Hidden Valley High School | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MEDRANO, Vanessa | Bassett | 2:58.51 | Robert Marshall Invitational |
| 2 | COFFLIN, Ellie | Christiansburg | 3:17.52 | Blue & Gold HS Invitational |
| 3 | DILLON, Sophie | Staunton River High School | 3:05.77 | Byrd Opener |
| 4 | THIERRY, Emma | William Byrd High School | 3:20.46 | Appomattox County High School In |
| 5 | TAYLOR, Charlotte | Alleghany | 3:05.73 | Spartan Relay Plus Warm Up |
| 6 | MOORE, Maddie | Lord Botetourt | 2:57.00 | Northside Invitational |
| 7 | GRUBB, Madesyn | Salem (Roanoke) | 3:00.00 | |
| 8 | FARAG, Sama | Blacksburg High School | 2:57.71 | Blacksburg Invitational |
| 9 | CHINN, Rose | Blacksburg High School | 3:11.84 | Blacksburg Invitational |
| 10 | BRUCE, Leighann | Christiansburg | 3:18.77 | Blacksburg Invitational |
| 11 | HENZEY, Isabelle | Salem (Roanoke) | 3:00.00 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BOWMAN, Kendall | Franklin County | 2:39.00 | |
| 2 | TANG, Julianna | Cave Spring High School | 2:47.24 | |
| 3 | DOWNS, Allie | Alleghany | 2:54.22 | Spartan Relay Plus Warm Up |
| 4 | BARONE, Lila | Hidden Valley High School | 2:44.40 | Northside Invitational |
| 5 | WALDRON, Riley | Staunton River High School | 2:45.82 | Byrd Opener |
| 6 | ROBERTS, Rebekah | Blacksburg High School | 2:38.00 | |
| 7 | WILSHER, Kaylee | Cave Spring High School | 2:41.24 | |
| 8 | OSBORNE, Abby | Franklin County | 2:49.00 | |
| 9 | HANCOCK, Addison | Salem (Roanoke) | 2:50.00 | |
| 10 | COOK, Brylynn | Staunton River High School | 2:52.42 | Byrd Opener |
| 11 | MEADOR, Mackenzie | Franklin County | 2:49.00 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | DAVIDSON, Maddie | James River (Buchanan) | 2:28.00 | |
| 2 | KREHBIEL, Skylar | Christian Heritage Academy | 2:36.53 | Blacksburg Invitational |
| 3 | COOK, Clara | Blacksburg High School | 2:33.76 | Blacksburg Invitational |
| 4 | ZHANG, Sadie | Blacksburg High School | 2:34.00 | |
| 5 | MORGAN, Willow | Alleghany | 2:36.27 | East Coast Elite VMI |
| 6 | WHITENACK, Lucy | William Byrd High School | 2:36.07 | Appomattox County High School In |
| 7 | KATZ, Emily | Blacksburg High School | 2:35.00 | |
| 8 | LARIMER, Kaylin | Christiansburg | 2:26.38 | |
| 9 | FLANDERS, Jazzy | Christian Heritage Academy | 2:33.26 | Blacksburg Invitational |
| 10 | HICKS, Alice | Cave Spring High School | 2:38.00 | |
| 11 | BRABY, Eliza | Patrick Henry (Roanoke) | 2:33.99 | Blue & Gold HS Invitational |