| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Halifax County | A | Easley | Lawson | Bellamy | Dean | 44.85 | 2024 Kellie Wells Classic |
| 4 | George Washington | A | Walker | Brandon | Pritchett | Newby | 44.19 | Rufus Knight Invitational (Forme |
| 5 | Gretna High School | A | Waller | Gunn | Younger | Chatten | 44.6h | Dogwood District Meet - Chatham/ |
| 6 | Halifax County | B | Lawson | Chambers | Dunkley | Ferrell | 45.85 |